The cryo-EM structure of E. coli CueR-TAC
September 28, 2020 "CueR activates transcription through a DNA distortion mechanism" Online: Nature Chemical Biology
August 12, 2020 "Structural basis for transcription inhibition by E. coli SspA " Online: Nucleic Acids Research
March 3, 2020 “RNA extension drives a stepwise displacement of an initiation-factor structural module in initial transcription”Online: PNAS; Bioart plant: link; Media report: CAS; SIPPE
December 30, 2019 “Crystal structures and biochemical analyses of the bacterial arginine dihydrolase ArgZ suggests a “bond rotation” catalytic mechanism.” Online: Journal of Biological Chemistry
December 17, 2019 “Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor” Online: eLife ; Media report: CAS; SIPPE
Transcription is the first and the most-regulated step of gene expression, by which RNA polymerase (RNAP) copies genetic information from DNA to RNA. It is fundamentally important to understand the structure, function, and regulation of the transcription machinery--RNAP. RNAPs are well conserved across bacterial species but are less conserved between bacteria and human. Therefore, bacterial RNAPs are promising drug target for antibiotic discovery. Two categories of bacterial RNAP inhibitors (Rifamycins and Fidaxomicin) are currently in clinical use for the treatment of various infectious diseases.
Our lab focuses on the molecular mechanism of transcription and transcription regulation. We also develop new bactericidal antibiotics targeting bacterial RNA polymerase. We employ multiple approaches in Structure biology (X-ray crystallography and cryo-EM), Biochemistry, Enzymology, Microbiology, and Bioinformatics. Our current projects include:
Molecular mechanism of bacterial transcription initiation
Transcription regulation by alternative initiator “sigma” factors
Molecular mechanism of transcription-coupled DNA repair
Molecular mechanism of bacterial transcription termination
Discovery and mechanism study of new RNAP regulatory proteins
Discovery of new antibiotics targeting bacterial RNA polymerase
Welcome our new lab manager, Zhi Yan joining our lab! Welcome new PhD students, Shanghai Dong, Guoqiang Li, Zhanxi Gu joining our lab!
Linlin You received the "Quanyin Innovation Talent Scholarship of 2019”. Congratulations!
Chengli Fang received the “UCAS National Graduate Scholoship of 2019”, Congratulations!
Chengzhi Yu received the “UCAS National Graduate Scholoship of 2019”, Congratulations!
Li Lingting received the Chinese government scholarship to fund her upcoming overseas study. Congratulations!
Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences, Shanghai, China
The Zhang Lab
| CAS Key Laboratory of Synthetic Biology | CAS Center for Excellence in Molecular Plant Sciences |
300 Fenglin Rd. Shanghai, China